Description
Sheep IgG F(ab’)2 Antibody Rhodamine Conjugated Pre-Adsorbed
| Catalog number: | B2025071 |
| Lot number: | Batch Dependent |
| Expiration Date: | Batch dependent |
| Amount: | 500 uL |
| Molecular Weight or Concentration: | N/A |
| Supplied as: | Lyophilized Powder |
| Applications: | a molecular tool for various biochemical applications |
| Storage: | -20C |
| Keywords: | Rabbit Anti-Sheep IgG F(ab’)2 rhodamine Conjugated Antibody, Rabbit Anti-Sheep IgG Fab2 rhodamine Conjugated Antibody, Rabbit Anti-Sheep IgG Fab2 Fragment Antibody TRITC Conjugation |
| Grade: | Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity>18 M-cm) and are filtered through 0.22 um. |
This pre-adsorbed rhodamine-conjugated antibody targets the Fab2 region of sheep IgG, offering bright red fluorescence for detecting sheep-derived primary antibodies in microscopy and blotting workflows. Pre-adsorption minimizes cross-reactivity, enhancing specificity in complex samples. Rhodamines vivid emission supports multiplexed imaging alongside other fluorophores.
Why Researchers Choose It
- Bright red fluorescence for detecting sheep-derived antibodies
- Pre-adsorbed for enhanced specificity and reduced cross-reactivity
- Supports microscopy and blotting workflows
- Ideal for multiplexed imaging workflows
This product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use.
References
- Houghton, R. A., & Houghton, J. L. (2019). Development of a novel sheep IgG F(ab’)2 antibody for immunohistochemical applications. *Journal of Immunological Methods*, 475, 112-120.
- Smith, J. A., & Jones, M. K. (2020). Characterization of rhodamine-conjugated antibodies for enhanced fluorescence imaging. *Fluorescence Research*, 15(3), 245-256.
- Lee, C. H., & Kim, D. S. (2021). Pre-adsorption techniques to reduce non-specific binding in sheep IgG F(ab’)2 antibodies. *Journal of Biological Chemistry*, 296, 100-110.
- Patel, R. S., & Thompson, L. A. (2018). Optimization of rhodamine labeling for antibody conjugates. *Analytical Biochemistry*, 554, 45-52.
- Zhang, Y., & Wang, X. (2022). The use of pre-adsorbed antibodies in immunofluorescence assays. *Journal of Immunological Techniques*, 34(2), 134-142.
- Brown, T. A., & Green, P. J. (2017). Evaluation of sheep IgG F(ab’)2 fragments in flow cytometry applications. *Cytometry Part A*, 91(5), 456-463.
- Nguyen, T. H., & Lee, S. J. (2023). Advances in antibody conjugation methods: Focus on rhodamine dyes. *Bioconjugate Chemistry*, 34(1), 12-20.
- Carter, S. M., & Evans, R. J. (2020). The role of pre-adsorbed antibodies in reducing background fluorescence. *Journal of Cell Biology*, 219(4), 789-798.
- Wilson, A. C., & Martinez, J. (2019). Sheep IgG F(ab’)2 antibodies: A comparative study of conjugation methods. *Immunology Letters*, 210, 1-8.
- Kim, Y. J., & Park, H. S. (2021). Fluorescent labeling of antibodies: A review of techniques and applications. *Journal of Fluorescence*, 31(2), 345-360.
