Description
His-Tag Purification Buffer Set
| Catalog number: | B2015581 |
| Lot number: | Batch Dependent |
| Expiration Date: | Batch dependent |
| Amount: | 1 kit |
| Molecular Weight or Concentration: | N/A |
| Supplied as: | Solution |
| Applications: | a molecular tool for various biochemical applications |
| Storage: | RT |
| Keywords: | Sodium Phosphate dibasic & Monosodium Phosphate |
| Grade: | Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity>18 M-cm) and are filtered through 0.22 um. |
References
- Eschenfeldt WH, Maltseva N, Stols L, Donnelly MI, Gu M, Nocek B, Tan K, Kim Y, Joachimiak A. Cleavable C-terminal His-tag vectors for structure determination J Struct Funct Genomics. 2010 Mar;11(1):31-9.
- Dhankher A, Lv W, Studstill WT, Champion JA. Coiled coil exposure and histidine tags drive function of an intracellular protein drug carrier J Control Release. 2021 Nov 10;339:248-258.
- Tonin F, Rosini E, Piubelli L, Sanchez-Amat A, Pollegioni L. Different recombinant forms of polyphenol oxidase A, a laccase from Marinomonas mediterranea Protein Expr Purif. 2016 Jul;123:60-9.
- Sheikholvaezin A, Blomberg F, Ohrmalm C, Sjsten A, Blomberg J. Rational recombinant XMRV antigen preparation and bead coupling for multiplex serology in a suspension array Protein Expr Purif. 2011 Dec;80(2):176-84.
- Akif M, Chauhan R, Mande SC. Expression, purification, crystallization and preliminary X-ray crystallographic studies of Mycobacterium tuberculosis thioredoxin reductase Acta Crystallogr D Biol Crystallogr. 2004 Apr;60(Pt 4):777-9.
- Myllykoski M, Kursula P. Expression, purification, and initial characterization of different domains of recombinant mouse 2,3-cyclic nucleotide 3-phosphodiesterase, an enigmatic enzyme from the myelin sheath BMC Res Notes. 2010 Jan 21;3:12.
- Schmidberger JW, Wilce JA, Weightman AJ, Wilce MC. Purification, crystallization and preliminary crystallographic analysis of DehI, a group I alpha-haloacid dehalogenase from Pseudomonas putida strain PP3 Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Jul 1;64(Pt 7):596-8.
- Majorek KA, Kuhn ML, Chruszcz M, Anderson WF, Minor W. Double trouble-Buffer selection and His-tag presence may be responsible for nonreproducibility of biomedical experiments Protein Sci. 2014 Oct;23(10):1359-68.
- Ronzetti MH, Baljinnyam B, Itkin Z, Jain S, Rai G, Zakharov AV, Pal U, Simeonov A. Application of temperature-responsive HIS-tag fluorophores to differential scanning fluorimetry screening of small molecule libraries Front Pharmacol. 2022 Nov 24;13:1040039.
