Description

Polystyrene Particles Conjugated to FITC (5 m) Catalog B2013345

Polystyrene Particles Conjugated to FITC (5 m) (Catalog B2013345) are brightly fluorescent green microspheres supplied as a 1 mL 1% aqueous suspension. These 5 m particles are covalently labeled with fluorescein isothiocyanate (FITC), providing strong green fluorescence (excitation ~495 nm, emission ~519 nm) that is fully compatible with standard FITC/GFP filter sets. They are excellent tools for fluorescence microscopy, flow cytometry calibration, phagocytosis studies, cellparticle interaction research, agglutination assays, and as traceable markers in diagnostic and nanomedicine applications.

Catalog number:	B2013345
Lot number:	Batch Dependent
Expiration Date:	Batch dependent
Amount:	1 mL
Molecular Weight or Concentration:	1% (w/v) suspension
Supplied as:	Aqueous suspension
Applications:	Fluorescence microscopy, flow cytometry, phagocytosis and cell interaction studies, agglutination assays, instrument calibration, particle tracking, and immunoassay development
Storage:	2-8C (do not freeze)
Keywords:	Polystyrene beads, FITC labeled, 5 m fluorescent microspheres, green fluorescent particles
Grade:	Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity>18 M-cm) and are filtered through 0.22 um.

Scientific Overview

These 5 m FITC-conjugated polystyrene particles combine a uniform microsphere core with bright, stable green fluorescence from covalently attached FITC. The larger 5 m size makes them particularly suitable for direct visualization under standard fluorescence microscopes, phagocytosis assays with macrophages or neutrophils, and as calibration standards in flow cytometry. The covalent labeling ensures minimal dye leakage, enabling reliable long-term tracking and quantitative studies.

Key applications include:

Fluorescence microscopy and live-cell imaging
Phagocytosis and immune cell interaction studies
Flow cytometry calibration and compensation
Agglutination and particle-based immunoassays
Cell tracking and biodistribution experiments

Usage & Handling Guidance

Store at 28C. Do not freeze. Gently vortex or pipette up and down before use to ensure homogeneous suspension. Dilute in PBS, culture medium, or assay buffer as required. Brief low-power sonication can be used if minor aggregation occurs after long-term storage.

Typical working concentration: 1010 beads/mL (optimize for assay)
Excitation/Emission: ~495 nm / ~519 nm (standard FITC/GFP filter sets)
Stability: Stable for months when stored refrigerated; protect from prolonged light exposure

What You Get

1 mL of 5 m Polystyrene Particles Conjugated to FITC (1% suspension)
Bright, photostable green fluorescence with covalent FITC labeling
Uniform particle size for reproducible experimental results
Ready-to-use aqueous suspension
For research use only (RUO)

Why Researchers Choose It

Bright FITC fluorescence compatible with standard laboratory equipment
5 m size ideal for microscopy, phagocytosis, and flow cytometry applications
Stable covalent conjugation minimizes dye leakage
Versatile tool for cell biology, immunology, and diagnostic assay development
High-quality biotechnology-grade particles with consistent performance

Frequently Asked Questions (FAQ)

What are the excitation and emission wavelengths?
Approximately 495 nm excitation and 519 nm emission (standard FITC channel).
Can these particles be used in phagocytosis studies?
Yes. The 5 m size is optimal for macrophage and neutrophil phagocytosis assays.
How should I store the suspension?
Store at 28C. Do not freeze. Mix gently before each use.
Are the particles surface-functionalized?
These are FITC-conjugated polystyrene particles. Additional surface chemistry options are available if further conjugation is needed.
What is the concentration?
1% (w/v) suspension. Dilute according to your experimental needs.

This product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.

References

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