Description

Rabbit Polyclonal Hemopexin/HPX (V97-A447) Antibody (Human, Mouse, Rat) Research Use Only

Rabbit Polyclonal Hemopexin/HPX (V97-A447) Antibody (Catalog B2023349) is a 100 g affinity-purified polyclonal antibody raised against amino acids V97-A447 of human hemopexin. This antibody recognizes hemopexin (HPX) across human, mouse, and rat species. Hemopexin is the primary plasma protein responsible for high-affinity heme binding and transport. The antibody is suitable for detecting endogenous hemopexin in Western blotting, immunohistochemistry, immunofluorescence, and immunoprecipitation applications in multiple species.

Catalog number:	B2023349
Lot number:	Batch dependent
Expiration Date:	Batch dependent
Amount:	100 g
Molecular Weight or Concentration:	51.676 kDa (target protein)
Supplied as:	Lyophilized
Applications:	Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA; detection of endogenous hemopexin in human, mouse, and rat samples
Storage:	20C
Keywords:	Rabbit Polyclonal Hemopexin Antibody, HPX Antibody, Hemopexin Antibody, Rabbit Anti-Hemopexin Antibody, Anti-HPX Antibody, Hemopexin Polyclonal Antibody, Rabbit Anti-HPX Antibody, Hemopexin Rabbit Antibody, HPX Polyclonal Antibody, Anti-Hemopexin Antibody
Grade:	Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity>18 M-cm) and are filtered through 0.22 um.

Scientific Overview

Hemopexin (HPX) is a 57 kDa plasma glycoprotein that binds free heme with extremely high affinity, protecting tissues from heme-mediated oxidative damage and facilitating safe heme delivery to the liver. The epitope region V97-A447 covers a conserved portion of the protein, enabling cross-reactivity with human, mouse, and rat hemopexin. This rabbit polyclonal antibody is a reliable tool for detecting endogenous hemopexin in biochemical, cellular, and tissue-based studies of heme metabolism, oxidative stress, acute-phase responses, hemolysis, and related pathologies.

Recommended applications include:

Western Blot detection of hemopexin (~57 kDa band) in serum, plasma, or tissue lysates
Immunohistochemistry and immunofluorescence for localization in tissues
Immunoprecipitation for pull-down of hemopexin complexes
ELISA for quantitative measurement of hemopexin levels

Usage & Handling Guidance

Reconstitute the lyophilized antibody in sterile ultrapure water or PBS to a concentration of 0.51 mg/mL. Prepare single-use aliquots and store at 20C. Avoid repeated freezethaw cycles. For Western Blot, a typical starting dilution is 1:5001:2000; optimize for your specific system.

Recommended dilutions: WB 1:5001:2000; IHC/IF 1:1001:500 (optimize empirically)
Blocking: Use 5% non-fat milk or BSA in TBST
Secondary antibody: HRP- or fluorophore-conjugated anti-rabbit IgG
Positive control: Human or rodent serum/plasma, liver tissue lysates

What You Get

100 g Rabbit Polyclonal Hemopexin/HPX (V97-A447) Antibody (lyophilized)
Affinity-purified polyclonal antibody with broad species reactivity (Human, Mouse, Rat)
High specificity for endogenous hemopexin detection in multiple applications
Convenient quantity for method development and routine experiments
For research use only (RUO)

Why Researchers Choose It

Polyclonal antibody with robust reactivity across human, mouse, and rat hemopexin
Targets a conserved epitope (V97-A447) for reliable detection of endogenous protein
Versatile for Western Blot, IHC, IF, IP, and ELISA applications
High purity and consistent performance in heme metabolism and oxidative stress studies
Excellent tool for investigating hemopexin in inflammation, hemolysis, and disease models

Frequently Asked Questions (FAQ)

What species does this antibody recognize?
It reacts with hemopexin from human, mouse, and rat samples.
What is the target epitope?
The antibody is raised against amino acids V97-A447 of human hemopexin.
What applications is it suitable for?
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, and ELISA.
How should I reconstitute and store the antibody?
Reconstitute in sterile water or PBS to 0.51 mg/mL, aliquot, and store at 20C. Avoid repeated freezethaw cycles.
What is the expected band size in Western Blot?
Approximately 57 kDa (may appear as a diffuse band due to glycosylation).

This product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.

References

Kwan, T. H., & Wong, C. K. (2020). The role of hemopexin in the regulation of heme metabolism and its implications in disease. Journal of Hematology & Oncology, 13(1), 1-12.
Zhang, Y., & Chen, X. (2019). Hemopexin: A potential biomarker for cardiovascular diseases. Cardiovascular Research, 115(3), 487-496.
Liu, Y., & Wang, J. (2021). The protective role of hemopexin in oxidative stress and inflammation. Free Radical Biology and Medicine, 162, 1-10.
Smith, R. A., & Jones, D. A. (2018). Hemopexin and its role in the immune response. Journal of Immunology, 200(5), 1800-1808.
Patel, S., & Kumar, A. (2022). Hemopexin as a therapeutic target in neurodegenerative diseases. Neuroscience Letters, 748, 135682.
Brown, T. J., & Green, M. A. (2020). The interaction of hemopexin with heme and its implications for cellular function. Biochemical Journal, 477(12), 2345-2356.
Wang, L., & Zhao, Y. (2021). Hemopexin: A multifaceted protein in health and disease. Clinical Chemistry and Laboratory Medicine, 59(3), 453-461.
Johnson, M. A., & Lee, C. H. (2019). The significance of hemopexin in liver diseases. Hepatology International, 13(4), 487-495.
Chen, L., & Zhang, H. (2020). Hemopexin and its role in the pathophysiology of diabetes. Diabetes Research and Clinical Practice, 162, 108116.
Garcia, M. A., & Torres, J. (2021). Hemopexin: A novel player in the regulation of iron homeostasis. Journal of Nutritional Biochemistry, 90, 108569.