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Animal Free Collagenase Neutral Protease

Original price was: $120.98.Current price is: $30.25.

SKU: PW14836854317 Category:

Description

Animal Free Collagenase Neutral Protease Catalog B2012710 (10 mg)

Catalog B2012710
Lot Batch dependent
Expiration Batch dependent
Amount 10 mg
Supplied As Lyophilized Powder
Applications Molecular tool combining collagenase and neutral protease for biochemical applications
Storage 28 C
Grade Biotechnology grade; highly pure; solutions made with Type I ultrapure water (>18 Mcm) and filtered through 0.22 m
Keywords Bacteria-Derived Animal Free Collagenase Neutral Protease Purified

Dual-enzyme system combining animal-free collagenase and neutral protease for high-fidelity extracellular matrix (ECM) digestion in RUO workflows. Animal Free Collagenase Neutral Protease (Catalog B2012710, 10 mg) is a lyophilized, biotechnology-grade reagent expertly formulated to deliver robust tissue dissociation while eliminating the risk of animal-derived contaminants. By merging Clostridium histolyticum collagenase and Bacillus polymyxa neutral protease in a single, highly pure package, it accelerates both collagen fiber breakdown and broad-spectrum proteolysisideal for complex biochemical and immunological protocols.

The animal-free formulation ensures traceability and compatibility with regenerative medicine, stem cell research, primary cell isolation, and other sensitive applications where animal-derived components must be avoided. The combination enzyme is particularly useful in workflows involving dense tissue types or protocols requiring sequential or simultaneous degradation of collagen and non-collagen matrix components. Its lyophilized format offers long shelf life, consistent activity, and flexibility in reconstitution and dosing.

Manufactured to high biotech standards, the enzyme blend is dissolved in ultrapure Type I water and filtered through 0.22 m, guaranteeing minimal impurities and reproducibility across lots. Whether used in histopathology preparation, ECM component extraction, or as a tool in proteomic sample prep, this reagent simplifies workflow complexity by consolidating two enzymatic activities into one high-performance, RUO-ready product. For labs scaling experimental throughput or validating robust protocols, custom bulk orders are also available to ensure uninterrupted access to this essential dual-enzyme solution.

Why researchers choose this product:

  • Animal-free formulation removes concerns about mammalian contaminants and variability
  • Dual-enzyme activity ensures efficient, comprehensive ECM digestion
  • Lyophilized, biotechnology-grade format facilitates flexible usage and extended stability
  • High purity and traceability support reproducibility in RUO workflows
  • Suitable for tissue dissociation, proteomic prep, primary cell isolation, and scale-up protocols

Indication for Use:
This product is for Research Use Only (RUO). It is not intended for diagnostic, therapeutic, or clinical applications.

References:

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  • 2: Szot GL, Lee MR, Tavakol MM, Lang J, Dekovic F, Kerlan RK, Stock PG, Posselt AM. Successful clinical islet isolation using a GMP-manufactured collagenase and neutral protease Transplantation. 2009 Sep 27;88(6):753-6.
  • 3: Brandhorst H, Johnson PR, Mnch J, Kurfrst M, Korsgren O, Brandhorst D. Comparison of Clostripain and Neutral Protease as Supplementary Enzymes for Human Islet Isolation Cell Transplant. 2019 Feb;28(2):176-184.
  • 4: Takahashi S, Simpser E. Granuloma collagenase and EDTA-sensitive neutral protease production in hepatic murine schistosomiasis Hepatology. 1981 May-Jun;1(3):211-20.
  • 5: Stull ND, Breite A, McCarthy R, Tersey SA, Mirmira RG. Mouse islet of Langerhans isolation using a combination of purified collagenase and neutral protease J Vis Exp. 2012 Sep 7;(67):4137.
  • 6: Cheung HS, Bohon S, Kozin F. Kinetics of collagenase and neutral protease release by neutrophils exposed to microcrystalline sodium urate Connect Tissue Res. 1983;11(1):79-85.
  • 7: Khiatah B, Tucker A, Chen KT, Perez R, Bilbao S, Valiente L, Medrano L, Rawson J, Forouhar E, Omori K, Kandeel F, Qi M, Al-Abdullah IH. Evaluation of collagenase gold plus BP protease in isolating islets from human pancreata Islets. 2018 Mar 4;10(2):51-59.
  • 8: Breite AG, Dwulet FE, McCarthy RC. Tissue dissociation enzyme neutral protease assessment Transplant Proc. 2010 Jul-Aug;42(6):2052-4.
  • 9: Dabbous MK, Roberts AN, Brinkley B. Collagenase and neutral protease activities in cultures of rabbit VX-2 carcinoma Cancer Res. 1977 Oct;37(10):3537-44.
  • 10: Wang H, Huang L, Wu L, Lan J, Feng X, Li P, Peng Y. The MMP-2/TIMP-2 System in Alzheimer Disease CNS Neurol Disord Drug Targets. 2020;19(6):402-416.