Description
Alkaline Phosphatase Conjugate Stabilizer
| Catalog number: | B2010384 |
| Lot number: | Batch-Dependent |
| Expiration date: | Batch-Dependent (3 years from date of manufacture) |
| Size: | 50 mL and bulk |
| Storage: | 28C |
| Other names: | AP Conjugate Stabilizer, AP Stabilizer, Alkaline Phosphatase (AP) conjugates Stabilizer, Protein-Free AP Stabilizer, AP Stabilizer Carrier-Free, Antibody-AP Stabilizer, Protein-AP Stabilizer |
| Application: | Uniquely efficient protein-free stabilizer for Alkaline Phosphatase conjugates (to proteins, antibodies and small molecules). Extensively validated in ELISA, CLIA, Western Blot and other immune and enzymatic assays. |
Alkaline Phosphatase Conjugate Stabilizer is a biotechnology-grade reagent formulated to preserve the enzymatic activity and structural integrity of alkaline phosphatase (AP) conjugates used in immunoassays. This stabilizer is ideal for extending the shelf life of AP-labeled antibodies and detection reagents in ELISA, Western blotting, and chemiluminescence platforms. It prevents denaturation, aggregation, and loss of enzymatic function during storage and repeated freeze-thaw cycles, ensuring consistent assay performance over time.
Researchers and assay developers working in diagnostics, bone metabolism studies, and enzyme-linked detection systems rely on AP conjugates for sensitive and specific signal generation. This stabilizer supports long-term reagent storage at 28C and integrates seamlessly into manual and automated workflows. Filtered through 0.22 m membranes and prepared with ultrapure water, it ensures minimal background interference and high reproducibility. Whether you’re formulating a new assay or maintaining existing kits, this stabilizer provides the reliability needed for robust and reproducible results.
Key benefits:
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Preserves enzymatic activity of alkaline phosphatase conjugates
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Prevents aggregation and degradation during storage and handling
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Compatible with ELISA, Western blotting, and chemiluminescence assays
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Filtered through 0.22 m membranes using ultrapure water
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Stored at 28C for long-term stability and reproducibility
Products are for in vitro research use only (RUO).
References
- Siller AF, Whyte MP. Alkaline Phosphatase: Discovery and Naming of Our Favorite Enzyme. J Bone Miner Res. 2018 Feb;33(2):362-364.
- Vimalraj S. Alkaline phosphatase: Structure, expression and its function in bone mineralization. Gene. 2020 Sep 5;754:144855.
- Lalls JP. Recent advances in intestinal alkaline phosphatase, inflammation, and nutrition. Nutr Rev. 2019 Oct 1;77(10):710-724.
- Azpiazu D, Gonzalo S, Villa-Bellosta R. Tissue Non-Specific Alkaline Phosphatase and Vascular Calcification: A Potential Therapeutic Target. Curr Cardiol Rev. 2019;15(2):91-95.
- Grote-Koska D, Klauke R, Brand K, Schumann G. Alkaline phosphatase activity pH impact on the measurement result. Clin Chem Lab Med. 2017 Jun 27;55(7):e146-e149.
- Pike AF, Kramer NI, Blaauboer BJ, Seinen W, Brands R. An alkaline phosphatase transport mechanism in the pathogenesis of Alzheimers disease and neurodegeneration. Chem Biol Interact. 2015 Jan 25;226:30-9.
- Serum alkaline phosphatase (AP) is perhaps the most widely used biomarker since it was discovered. Introduction. Subcell Biochem. 2015;76:v-vii.
- Niu X, Ye K, Wang L, Lin Y, Du D. A review on emerging principles and strategies for colorimetric and fluorescent detection of alkaline phosphatase activity. Anal Chim Acta. 2019 Dec 4;1086:29-45.
- Brady JJ, McGoldrick D, OCallaghan K, McNamara F, Mulready KJ, Cullen MR, Denieffe S, Fitzgibbon M. Bone alkaline phosphatase on the IDS-iSYS automated analyser; cross-reactivity with intestinal ALP. Clin Chem Lab Med. 2019 Jul 26;57(8):e186-e188.
