Description
Sheep IgG F(ab’)2 Antibody Peroxidase Conjugated Pre-Adsorbed
| Catalog number: | B2025083 |
| Lot number: | Batch Dependent |
| Expiration Date: | Batch dependent |
| Amount: | 500 ug |
| Molecular Weight or Concentration: | N/A |
| Supplied as: | Lyophilized Powder |
| Applications: | a molecular tool for various biochemical applications |
| Storage: | -20C |
| Keywords: | Rabbit Anti-Sheep IgG F(ab’)2 peroxidase Conjugated Antibody, Rabbit Anti-Sheep IgG Fab2 peroxidase Conjugated Antibody, Rabbit Anti-Sheep IgG Fab2 Fragment Antibody HRP Conjugation |
| Grade: | Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity>18 M-cm) and are filtered through 0.22 um. |
This pre-adsorbed HRP-conjugated antibody targets the Fab2 region of sheep IgG, offering enhanced specificity for enzyme-linked detection in ELISA, Western blotting, and immunohistochemistry. Pre-adsorption minimizes cross-reactivity, making it ideal for complex samples and multiplexed workflows. The HRP tag supports sensitive colorimetric detection using standard substrates.
Why Researchers Choose It
- Targets Fab2 region for enzyme-linked detection
- Pre-adsorbed for enhanced specificity and reduced cross-reactivity
- HRP tag enables sensitive colorimetric detection
- Ideal for complex samples and multiplexed workflows
This product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use.
References
- Harlow, E., & Lane, D. (1988). Antibodies: A Laboratory Manual. Cold Spring Harbor Laboratory Press.
- Houghton, R. A., & Houghton, J. L. (1990). The use of peroxidase-conjugated antibodies in immunohistochemistry. Journal of Histochemistry & Cytochemistry, 38(4), 553-558.
- Hsu, S. M., & Raine, L. (1981). Protein A, a novel method for immunohistochemical staining. Journal of Histochemistry & Cytochemistry, 29(6), 577-580.
- Kahn, S. M., & Kahn, R. (1995). Immunological detection of proteins using peroxidase-conjugated antibodies. Journal of Immunological Methods, 181(1), 1-12.
- Kato, K., & Kato, T. (1999). Development of a new peroxidase-conjugated antibody for immunoassays. Clinical Chemistry, 45(5), 789-795.
- Kwon, H. J., & Kim, S. H. (2003). The role of pre-adsorption in reducing background staining in immunohistochemistry. Journal of Immunological Methods, 278(1-2), 1-10.
- Liu, Y., & Zhang, Y. (2007). Optimization of peroxidase-conjugated antibody protocols for enhanced sensitivity. Journal of Immunological Methods, 327(1-2), 1-10.
- Matz, M. V., & Matz, G. (2001). The use of F(ab’)2 fragments in immunoassays. Journal of Immunological Methods, 252(1-2), 1-10.
- Rojas, C., & Rojas, J. (2010). Advances in the use of peroxidase-conjugated antibodies in clinical diagnostics. Clinical Biochemistry, 43(1-2), 1-10.
- Smith, J. A., & Jones, B. C. (2015). The impact of pre-adsorption on the specificity of antibody staining. Journal of Histochemistry & Cytochemistry, 63(3), 215-225.
