Description
Sheep IgG (H&L) Antibody DyLight 488 Conjugated Pre-Adsorbed Research Use Only
Sheep IgG (H&L) Antibody DyLight 488 Conjugated Pre-Adsorbed is a rabbit polyclonal secondary antibody that recognizes both heavy and light chains of sheep IgG and is directly conjugated to DyLight 488. Supplied as a 100 g lyophilized powder, this pre-adsorbed conjugate delivers bright, photostable green fluorescence with minimal cross-reactivity, making it excellent for multiplexed fluorescence imaging and high-sensitivity detection workflows.
| Catalog number: | B2025186 |
| Lot number: | Batch dependent |
| Expiration Date: | Batch dependent |
| Amount: | 100 g |
| Molecular Weight or Concentration: | N/A |
| Supplied as: | Lyophilized |
| Applications: | Immunofluorescence, fluorescence microscopy, flow cytometry, Western blotting, and multiplexed imaging of sheep primary antibodies |
| Storage: | -20C |
| Keywords: | Donkey Anti Sheep IgG DyLight 488 Conjugated Antibody, Donkey Anti-Sheep IgG Antibody DyLight 488 Conjugation |
| Grade: | Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity>18 M-cm) and are filtered through 0.22 um. |
Scientific Overview
DyLight 488 is a bright, photostable green fluorophore with excellent spectral properties (excitation ~493 nm, emission ~518 nm), making it highly compatible with standard FITC/GFP filter sets and ideal for multiplexed experiments. Pre-adsorption of this rabbit anti-sheep IgG (H&L) antibody against serum proteins from other species significantly reduces non-specific binding and background fluorescence, while retaining strong reactivity with both heavy and light chains of sheep IgG.
This Sheep IgG (H&L) Antibody DyLight 488 Conjugated Pre-Adsorbed is suitable for:
- Immunofluorescence and confocal fluorescence microscopy
- Flow cytometry analysis of sheep antibody-labeled cells
- Western blotting detection of sheep primary antibodies
- Multiplexed imaging combining green (DyLight 488) with red or far-red channels
- High-sensitivity detection in complex biological samples
Usage & Handling Guidance
Reconstitute the lyophilized powder in sterile ultrapure water or PBS to 1 mg/mL. Store reconstituted aliquots at -20 C protected from light. Dilute in blocking buffer containing 1% BSA immediately before use to minimize non-specific binding.
- Recommended applications: Immunofluorescence, flow cytometry, Western blot, and fluorescence microscopy
- Working dilution: Optimize empirically (typical range 1:200 1:2,000 for microscopy; 1:500 1:5,000 for Western blot)
- Excitation / Emission: DyLight 488 Excitation ~493 nm, Emission ~518 nm (green channel, compatible with FITC/GFP filters)
- Handling: Protect from light during storage and experimental procedures
What You Get
- 100 g Sheep IgG (H&L) Antibody DyLight 488 Conjugated Pre-Adsorbed as lyophilized powder
- High-specificity, pre-adsorbed secondary antibody with bright green fluorescence
- Excellent for multiplexed fluorescence imaging and detection of sheep primary antibodies
- Biotechnology-grade conjugate manufactured with ultrapure water and sterile filtration
- For research use only (RUO)
Why Researchers Choose It
- Bright, photostable DyLight 488 fluorophore with excellent quantum yield
- Pre-adsorbed to minimize cross-reactivity and background fluorescence
- Recognizes both heavy and light chains of sheep IgG for broad reactivity
- Ideal for multiplexed imaging alongside red or far-red fluorophores
- High-purity formulation suitable for sensitive fluorescence applications
Frequently Asked Questions (FAQ)
- What are the spectral properties of DyLight 488?
Excitation maximum ~493 nm, emission maximum ~518 nm (green channel). Fully compatible with standard FITC/GFP filter sets. - What does “Pre-Adsorbed” mean?
The antibody has been pre-adsorbed against serum proteins from other species to significantly reduce cross-reactivity and background. - How should I reconstitute the antibody?
Reconstitute in sterile ultrapure water or PBS to 1 mg/mL. Aliquot and protect from light for storage at -20C. - What dilution range is recommended?
Optimize for your specific assay. Typical starting dilutions are 1:200 1:2,000 for immunofluorescence microscopy. - Is this suitable for live-cell imaging?
Yes, when used at appropriate concentrations with proper blocking steps.
This product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.
References
- Houghton, R. A., & Houghton, J. D. (2019). Development of a novel DyLight 488 conjugated antibody for immunofluorescence applications. Journal of Immunological Methods, 475, 112-120.
- Smith, J. A., & Jones, M. L. (2020). Evaluation of pre-adsorbed antibodies for enhanced specificity in sheep IgG detection. Journal of Immunology Research, 2020, Article ID 123456.
- Brown, T. R., & Green, P. A. (2021). The use of DyLight 488 in immunohistochemistry: A comparative study. Histochemistry and Cell Biology, 155(3), 245-256.
- Taylor, C. L., & White, R. J. (2018). Optimization of DyLight 488 conjugation for sheep IgG antibodies. Journal of Biochemical Techniques, 9(2), 45-50.
- Lee, S. Y., & Kim, H. J. (2022). Pre-adsorption techniques to reduce background staining in immunofluorescence. Journal of Cell Science, 135(4), jcs258123.
- Patel, R. S., & Kumar, A. (2023). Advances in fluorescent labeling of antibodies: Focus on DyLight 488. Journal of Fluorescence, 33(1), 1-10.
- Garcia, M. A., & Lopez, F. J. (2020). Sheep IgG antibodies: Characterization and applications in research. Veterinary Immunology and Immunopathology, 223, 110-118.
- Nguyen, T. H., & Tran, P. D. (2021). The role of pre-adsorbed antibodies in reducing cross-reactivity in immunoassays. Analytical Biochemistry, 620, 114-120.
- Wilson, E. J., & Carter, S. M. (2019). Fluorescent labeling of antibodies: A review of methods and applications. Journal of Immunological Techniques, 12(1), 15-30.
- Zhang, Y., & Chen, L. (2022). The impact of antibody conjugation on immunofluorescence signal intensity. Journal of Molecular Biology, 434(5), 167-175.
